Immunoassay, monoclonal antibody, and hybridoma
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United States of America Patent
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Jun 11, 1996
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N/A
app pub date -
Apr 27, 1993
filing date -
Aug 9, 1991
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Abstract
The present invention relates to a method for determining a lipophilic compound, comprising using an antibody tolerant to one or more organic solvents, and carrying out an antigen-antibody reaction in the presence of one or more organic solvents. By the present method, it is possible to perform an antigen-antibody reaction of the lipophilic compound and the antibody, using an extract of a sample by one or more organic solvents, and thus the procedure becomes simple. Further, the present invention also relates to a monoclonal antibody specific to at least okadaic acid, dinophysistoxin-1 and dinophysistoxin-3, a hybridoma which produces the above monoclonal antibody, and an immunological determination method of diarrheal shellfish poisons using the above monoclonal antibody. According to the present invention, specific determination of all the three types of diarrheal shellfish poisons becomes possible.
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