Cloned pullulanase
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United States of America Patent
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May 7, 1996
Issued Date -
N/A
app pub date -
Dec 28, 1994
filing date -
Oct 5, 1993
priority date (Note) -
Expired
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Abstract
A method is disclosed for the expression of an active pullulanase enzyme in a microorganism host. In one aspect, a DNA construct contains a sequence encoding the pullulanase enzyme, except for the nucleotides necessary to encode the first two amino acids in mature pullulanase, and regulating sequences allowing expression of the coding sequence in a microorganism host. An advantageous DNA construct contains regulatory sequences permitting expression of the pullulanase in a yeast cell.
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