Enhancement of polynucleotide hybridization
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United States of America Patent
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Apr 30, 1996
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N/A
app pub date -
Sep 2, 1993
filing date -
Jul 1, 1991
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Expired
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Abstract
Hybridization buffers, for hybridizing complementary polynucleotides, contain polyvinyl alcohol (MW 1000-20000) and/or polystyrene sulphonic acid (e.g. MW 60000-80000) as a rate enhancer, generally at a concentration of 1-10%. Dextran sulphate, polyethylene glycol and cationic detergents may be additionally present. The method is useful when one of the two complementary polynucleotides is immobilised, or is in in situ hybridizations.
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