Methods of identifying lymphocytes with mutator phenotypes
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United States of America Patent
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Jan 9, 1996
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N/A
app pub date -
Jan 14, 1994
filing date -
Jan 14, 1994
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Abstract
This invention provides methods for identifying lymphocytes with a mutator phenotype by isolating lymphocytes bearing a selectable marker; identifying among the lymphocytes a population belonging to the same clonally amplified set; and determining whether the population has at least two lymphocytes having, between them, two independent mutations among different alleles at the gene locus of the selectable marker; the presence of which indicates a mutator phenotype. In one embodiment of the invention, the selectable marker is hprt.sup.-.
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