Method of preparing polypeptides in cell-free translation system
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United States of America Patent
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Dec 26, 1995
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app pub date -
Dec 16, 1992
filing date -
Dec 21, 1988
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Abstract
Cell-free production of protein is provided by employing a barrier which defines a reaction region for maintaining the high molecular weight components of the cell-free system while providing means for replenishing loss of low molecular weight components and removing low molecular weight products. Various configurations may be employed for continuous removal and addition of material during the polypeptide synthesis. Conveniently, porous membranes, hollow fibers, or other barriers may be employed. High ratios of peptide to RNA template result.
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