Modified thermo-resistant DNA polymerases
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United States of America Patent
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Dec 12, 1995
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N/A
app pub date -
Nov 23, 1993
filing date -
Nov 23, 1993
priority date (Note) -
Expired
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Abstract
Novel, modified Taq DNA polymerases and genes encoding for them are disclosed. The modified Taq DNA polymerases of the invention are the same size, have the same heat stability and synthesis rate as the native enzyme, but lack the 5'-3' exonuclease activity. As a result of this modification, the enzymes have improved processivity as compared to the native enzyme. The enzymes of the present invention enable improved methods of conducting PCR, DNA sequencing, and DNA synthesis.
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