Multilayer analytical element for salicylate assay
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United States of America Patent
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Dec 12, 1995
Grant Date -
N/A
app pub date -
Mar 25, 1994
filing date -
Mar 25, 1994
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A multilayer analytical element for measuring salicylate is disclosed. The element has a support. The support is coated with the following layers, top down to the support: a) a spreading layer, b) a dye layer having a pH greater than 6.5 and comprising tyrosinase and a water-insoluble hydrazone coupling agent, c) a barrier layer that prevents passage of molecules having a molecular weight in excess of 5,000, and d) a reagent layer comprising salicylate hydroxylase and nicotinamide adenine dinucleotide (NADH), wherein the spreading layer comprises valproic acid, 8-anilino-1-naphthalenesutfonic acid (ANS) or the magnesium salt of 8-anilino-1-naphthalenesulfonic acid.
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