Method for preparing immobilized enzyme conjugates and immobilized enzyme conjugates prepared thereby
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United States of America Patent
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Dec 5, 1995
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N/A
app pub date -
Jan 9, 1995
filing date -
Sep 1, 1993
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Abstract
A method for preparing an immobilized enzyme conjugate, whereby the enzyme is treated with a polyfunctional amine reactive material for forming a treated enzyme-containing adduct before being immobilized on a solid support which has been contacted with a solution of a polyamine compound. The method is especially preferred for use with glucoamylase, fungal .alpha.-amylase and .beta.-amylase. Immobilized enzyme conjugates formed by use of this method include treated enzyme-containing adducts. The immobilized enzyme conjugates disclosed herein are more stable and the enzymes immobilized therein are more tightly-held than those otherwise obtained and provided.
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