Particle-mediated transformation of mammalian unattached cells
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United States of America Patent
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Nov 28, 1995
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Apr 2, 1993
filing date -
Oct 15, 1991
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Abstract
A method of genetically transforming mammalian unattached cells is disclosed. The method begins by preparing copies of a nucleic acid construct and coating these copies onto biologically inert carrier particles. Mammalian unattached cells are isolated in a liquid suspension. The cell suspension is placed on a target surface, wherein the liquid is spread to a thin film on the target surface. In an alternative embodiment of the present invention, the liquid is spread onto a porous surface. The cells are bombarded with the construct-coated particles in such a fashion that some particles lodge in the interior of at least some of the cells. The existence and expression of the construct within the cell is verified.
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