Protein ligand binding region mapping system
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United States of America Patent
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Nov 7, 1995
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N/A
app pub date -
Mar 31, 1993
filing date -
Mar 31, 1993
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Abstract
A method for determining the nucleic acid sequence that encodes ligand binding domain on any protein encoded by a known gene includes the steps of non-specifically cleaving the coding region of the known gene, size-selecting the cleavage products, cloning the size-selected gene products into an expression vector active in a heterologous host, using a novel cloning strategy expressing the peptides produced by the clones and screening for clones that produce peptides that interact with an antibody or ligand of interest.
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