Electrophoresis in low conductivity buffers
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United States of America Patent
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Nov 7, 1995
Grant Date -
N/A
app pub date -
Jan 30, 1995
filing date -
Jan 30, 1995
priority date (Note) -
Expired
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Abstract
Electrophoresis is performed in buffers which exhibit both substantial buffering capacity and low electrical conductivity, permitting the separations to be performed at high field strengths without loss of resolution. Four classes of buffering agents are cited as examples: (1) buffering agents with a small number of charged groups per molecule; (2) carrier ampholytes fractionated to a narrow isoelectric point range; (3) low molecular weight buffering ampholytes with an isoelectric point which is close in value to one of the pK values of the ampholyte; and (4) high molecular weight buffering ampholytes in which the acidic and basic groups have the same or very close pK values. These buffering agents are of particular interest in capillary electrophoresis.
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