M-RNA purification
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United States of America Patent
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Oct 17, 1995
Issued Date -
N/A
app pub date -
May 2, 1994
filing date -
Jul 19, 1991
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Abstract
A method kit for purification of m-RNA from a cell are disclosed. Guanidine containing moieties, at high molarity, are use to quickly lyse the cell. They also act to inhibit RNase activity. Without the need for isolation of total RNA, the lysate can then be directly purified from the lysate using oligo dT (or U) by reducing the guanidine concentration via a dilution step.
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