Standard platelet samples and method for preparation thereof
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United States of America Patent
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Oct 10, 1995
Grant Date -
N/A
app pub date -
Apr 22, 1992
filing date -
Apr 22, 1992
priority date (Note) -
Expired
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Abstract
A method for determining the activation level of platelets and other types of blood cells that undergo activation by ELISA and other immunoassay techniques includes the step of reacting a sample containing the blood cells in a liquid phase with an excess quantity of an activation-specific primary antibody prior to allowing the cells in the sample to bind to a solid surface. This differs from typical ELISA procedures, wherein the cells are bound to the surface of a plastic test tube or microtiter plate prior to addition of the primary antibody. It has been discovered according to the invention that binding the cells to a surface prior to reaction with the primary antibody changes the activation level of the cells, making the assay less accurate.
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