Method of preparing an active human neutrophil chemotactic factor polypeptide
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United States of America Patent
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Mar 28, 1995
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Mar 23, 1992
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Aug 29, 1988
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Abstract
A simple and efficient method of preparing active recombinant human neutrophil chemotactic factor. The human neutrophil chemotactic factor (NCF) is solubilized from a transformant cell homogenate using urea or guanidine hydrochloride. The polypeptide is further purified from the solution using conventional protocols such as CM-Sepharose CL-6B, HW-55 column, etc.. By using this method, large amounts of active human NCF can be recovered and purified.
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