Method for purifying factor VIII and preparations obtained
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United States of America Patent
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Dec 6, 1994
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N/A
app pub date -
Sep 23, 1992
filing date -
Sep 26, 1991
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Expired
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Abstract
In the method for purifying factor VIII from cryoprecipitate, which is dissolved and then treated with alumina gel, the extract is diluted to a protein concentration not exceeding approximately 5 g/l and subjected to viral inactivation with solvent/detergent, the inactivated extract containing the solvent/detergent is then subjected to chromatography on a weak anion exchange column which is hydrophilic in nature and factor VIII is then eluted with a dissociating buffer.
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