Method of preparing human conjunctival mast cells for mast cell stabilization assays

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United States of America Patent

PATENT NO 5360720
SERIAL NO

08134148

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Abstract

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By allowing the mast cell enriched population of human conjunctival tissue cells to incubate for a minimum of about forty (40) hours post enzymatic digestion, the treatment window between spontaneous histamine release and anti-human IgE stimulated histamine release is increased. Culturing the human conjunctival tissue mast cells decreases the spontaneous release of histamine and increases the anti-IgE stimulated histamine release, greater than ten fold over spontaneous, at time points over forty (40) hours. This treatment window is sufficient to detect a compound's stabilizing or anti-allergic activity.

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ALCON LABORATORIES INC6201 SOUTH FREEWAY FORT WORTH TEXAS 76134 UNITED STATES OF AMERICA

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Click to zoom InYear of Issuance% of Matters IssuedCumulative IssuancesYearly Issuances199319941995199619971998199920002001200220032004200520062007200820092010201120120255075100

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Miller, Steven T Arlington, TX 42 335
Yanni, John M Burleson, TX 98 1348

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  • 5 Citation Count
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Citation count rangeNumber of patents cited in rangeNumber of patents cited in various citation count ranges47636188111401 - 1011 - 2021 - 3031 - 4051 - 6071 - 8091 - 100100 +05101520253035404550556065707580

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