Agrobacterium radiobacter K84 carrying transfer-deficient pAgK84 plasmids
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United States of America Patent
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Oct 11, 1994
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May 26, 1992
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Oct 20, 1987
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Abstract
The present invention provides transfer-deficient pAgK84 plasmids, non-pathogenic strains of Agrobacterium radiobacter K84 carrying such plasmids, and a method useful for control of crown gall disease mediated by A. tumefaciens. The plasmids include genes encoding the synthesis of the antibiotic agrocin 84 and are modified in the transfer region of the plasmid by sufficient deletion to inhibit transfer of the plasmid. The plasmids are used to develop non-pathogenic strains of A. radiobacter K84 that stably produce agrocin 84 and inhibit the development of crown gall disease.
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