Method for the determination of the concentration of an enzyme substrate and a sensor for carrying out the method
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United States of America Patent
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Aug 23, 1994
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N/A
app pub date -
Mar 29, 1993
filing date -
Aug 24, 1988
priority date (Note) -
Expired
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Abstract
A method for the continuous and reversible determination of the concentration of an enzyme substrate such as glucose in an specimen, wherein the specimen is brought into contact with a corresponding enzyme selected from oxidases and oxygenases and to which a flavin coenzyme (FMN, FAD) is bonded, the flavin coenzyme changing to a reduced form by the enzyme substrate and to an oxidized form by molecular oxygen dissolved in the specimen, and the change in the fluorescence spectrum, produced by means of the reduction of the flavin coenzyme, is measured.
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