Luminescence monitoring with modulation frequency multiplexing
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United States of America Patent
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May 31, 1994
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app pub date -
Jan 29, 1992
filing date -
Feb 16, 1990
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Abstract
A luminescence based analytical probe is excited with light varying repetitively in amplitude at multiple modulation frequencies. A composite signal incorporating components at a plurality of these modulation frequencies, produced by emissions in all of the various decaying modes is analyzed to provide information, such as the fractional luminescence contribution and/or lifetime of each individual decaying mode because the various decaying mode are segregated by analysis of the composite signal, there is no need to segregate light from the various decaying mode according to the wavelength of the light. Therefore, the probe may incorporate plural luminescent materials which have different decay modes with different luminescent decay times and sensitive to different conditions to be monitored, even if the plural luminescent materials absorb and emit light at the same wavelengths.
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