Mass propagation through shoot primordia and regeneration of plants from protoplasts of shoot primordia
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United States of America Patent
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May 10, 1994
Issued Date -
N/A
app pub date -
Jul 22, 1992
filing date -
Jun 26, 1986
priority date (Note) -
Expired
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Abstract
A process for the mass propagation of a woody plant comprising the steps of removing a shoot tip from the woody plant, transplanting the shoot tip to an artificial medium containing inorganic salts and plant growth hormones as main ingredients, and rotary-culturing the shoot tip under illumination to form shoot primordia, and stationary-culturing the shoot primordia in a liquid medium to regenerate shoots. Further, a process for the regeneration of plants comprising the steps of preparing a shoot primordium by rotary-culturing a shoot tip of a plant, treating the shoot primordium with at least one enzyme to prepare a protoplast, culturing the protoplast in an artificial medium containing plant hormones and inorganic and organic salts as main ingredients to form a callus, and incubating the callus in a regeneration medium to regenerate a shoot; plus intermediate materials of the process, and processes for production of the intermediate materials.
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