Installation for obtaining plasmids and cosmids
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United States of America Patent
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Apr 19, 1994
Issued Date -
N/A
app pub date -
Nov 13, 1992
filing date -
Jun 21, 1988
priority date (Note) -
Expired
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Abstract
A process of separating plasmids and cosmids from bacterial cells in which they are contained, comprising adding cultured bacterial cells separated from a culture medium to an inclusion gel-forming substance; lysing the bacterial cells; solubilizing the thus lysed bacterial cells; solidifying the inclusion gel-forming substance to form a gel either before or after the step of solubilizing; and applying an electric field to the thus formed gel sufficient to cause electrophoretic migration of the plasmids and cosmids and isolation of the plasmids and cosmids. Also disclosed is a device for carrying out the process.
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