Plant thioesterase having preferential hydrolase activity toward C12 acyl-acp substrate
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United States of America Patent
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Apr 19, 1994
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Nov 10, 1992
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Apr 26, 1990
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Abstract
This invention relates to a novel class of plant enzymes, thioesterases having preferential hydrolase activity toward one or more medium-chain acyl-ACP substrates. Purification, especially the removal of substantially all other plant proteins, and use of the plant thioesterase is provided, including the use of the protein as a tool in gene isolation for biotechnological applications and a description of various uses for such nucleic acid sequences thereto. The enzyme is preferrably obtained from Umbellularia californica and has preferential hydrolase activity toward C12 acyl-ACP substrate.
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