Method for separation of .beta.-amylase
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United States of America Patent
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Mar 15, 1994
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N/A
app pub date -
Nov 10, 1992
filing date -
Feb 7, 1992
priority date (Note) -
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Abstract
Disclosed is a method of separating .beta.-amylase from a solution containing .beta.-amylase, in which the solution is treated with an .alpha.-cyclodextrin fixed water-insoluble high polymer compound in the presence of ammonium sulfate to form an adsorbed composite of the high polymer compound and .beta.-amylase and soluble impurities not adsorbed to the high polymer compound are separated and removed. By the method, a high-purity .beta.-amylase, which is highly useful in the starch saccharification industry, may specifically and efficiently be isolated from a .beta.-amylase-containing mixture solution.
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