Bioluminescence method for the determination of pesticides
Number of patents in Portfolio can not be more than 2000
United States of America Patent
Stats
-
Feb 1, 1994
Issued Date -
N/A
app pub date -
Jan 9, 1992
filing date -
Jul 19, 1990
priority date (Note) -
Expired
status (Latency Note)
Importance
Abstract
A method for the rapid and sensitive determination of organophosphate and carbamate pesticides is disclosed. The method employs an insect brain preparation having a mixture of receptors or enzymes with sites that interact with the organophosphate and carbamate pesticides. The pesticides alter or reduce brain activities of the insect brain preparation which are inversely correlated with pesticide concentration in a test sample. The activity is measured by employing substrates which upon exposure to the insect brain preparation are structurally altered and a light emission reaction is observed. The substrates used are 6-substituted D-Luciferin esters wherein the D-Luciferin ester in the presence of the insect brain preparation is inhibited during hydrolysis while in the presence of the pesticide to be determined. Further the D-Luciferin ester in the presence of the insect brain preparation reacts to cleave the substituted ester group at the 6-position to produce D-Luciferin for further reaction with ATP and luciferase to produce bioluminescence. The bioluminescence can then be measured and compared with a control in order to determine the concentration of a pesticide in the test sample.
First Claim
Family
International Classification(s)
Cited Art Landscape
Patent Citation Ranking
Recipient Email Address
Recipient Email Address
Comment
Recipient Email Address
Add to Portfolio(s)
To add this patent to one, or more, of your portfolios, simply click the add button.
This Patent is in these Portfolios:
Add to additional portfolios:
Last Refreshed On:
Changes done successfully