Exopeptidase catalyzed site-specific bonding of supports, labels and bioactive agents to proteins
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United States of America Patent
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Jan 18, 1994
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N/A
app pub date -
Jun 30, 1989
filing date -
Jun 30, 1989
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Expired
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Abstract
The invention provides a means for attaching a label, support or bioactive agent to a protein with an exopeptidase at a site that is remote from the active site of the protein. More specifically the invention is directed to a method for the attachment of an amino acid, amine and alcohol nucleophile to the carboxyl terminus of a protein. In one embodiment, a labeled nucleophile is attached to a protein such as an antibody. In other embodiments, the invention is directed to a method for the attachment of a protein to an immobilization support and to a method for the attachment of a bioactive agent to a protein.
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