Recombinant herpesviruses, in particular for the production of vaccines, process for preparing them, plasmids produced during this process and vaccines obtained
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United States of America Patent
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Nov 30, 1993
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N/A
app pub date -
Jan 3, 1992
filing date -
Mar 12, 1990
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Abstract
The recombinant virus chosen from the turkey herpesvirus(HVT), Aujeszky's disease virus (PRV), infectious bovine rhinotracheitis virus (BHV) feline infectious rhinotracheitis virus (FHV), equine rhinopneumonia virus (EHV), canine herpesvirus (CHV), duck herpesvirus, human simplex herpesvirus (HSV), varicella virus (VZV), Epstein-Barr virus (EBV), cytomegalovirus (CMV), comprises at least one heterologous gene, coding particularily for a viral, bacterial or parasitic immunogen inserted in the region of the genome of the said virus corresponding to the gene of the small sub-unit RR2 of ribonucleotide reductase so that it may be expressed. The method for preparing a virus of this type involves inserting the heterlogous gene into the said region of the genome, particularly after partial or total deletion of this region. The recombiant virus obtained may be used particularly for preparing living vaccines. The small sub-unit of the ribonucleotide reductase of the turkey herpesvirus (HVT) has been sequenced.
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