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Abstract

Method of fermentative production and isolation of Cyclosporin A by aerobic fermentation of a fungus microorganism in a medium containing mineral salts, a nitrogen-containing substrate, including using a producer strain consisting of strain Wb 6-5 of Tolypocladium inflatum, IMET 43 899 Sesquicilliopsis rosariensis G.ARNOLD, IMET 43 888, Sesquicilliopsis rosariensis G.ARNOLD F605, IMET 43 887, a mutant thereof or a strain obtained therefrom by selection; culturing the producer strain in a culture medium containing an ammonium salt as a single source of nitrogen; mixing the culture medium with a filter aid to form a suspension; after the mixing step, filtering the suspension to form a filter cake containing moist biomass and filter aid; drying the filter cake after the filtering; after the drying step, extracting the filter cake with a supercritical gas, advantageously carbon dioxide, or a lower carboxylic acid ester to form an extract; distributing the extract between aqueous methanol solution and petroleum ether to remove fats and form a purified extract; and chromatographing the purified extract by preparative HPLC using silica gel or aluminum oxide.

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