Glassified restriction enzymes
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United States of America Patent
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Oct 5, 1993
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app pub date -
Sep 20, 1991
filing date -
Sep 20, 1991
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Abstract
A room temperature stable restriction enzyme in a glassified carbohydrate stabilizer is disclosed. A restriction enzyme reaction buffer containing Mg.sup.+2 is dispersed in the stabilizer. One cleaves DNA merely by adding water and DNA to the composition. The composition can reduce glycerol-caused star activity by avoiding the need for glycerol. The composition also reduces star activity caused by high concentrations of enzymes. Another method is provided of comparing cleaved DNA samples in connection with forensic and paternity testing applications.
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