Sensing surfaces capable of selective biomolecular interactions, to be used in biosensor systems
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United States of America Patent
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Sep 7, 1993
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app pub date -
May 10, 1991
filing date -
Nov 10, 1988
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Abstract
A sensing surface suitable for use in a biosensor, comprising: a film of a free electron metal selected from the group consisting of copper, silver, aluminum and gold; and a densely packed monolayer of an organic molecule X-R-Y coated on one of the faces of said film where X is a group selected from the group consisting of asymmetrical or symmetrical disulfide (--SSR'Y', -SSRY, sulfide (-SR'Y', -SRY), diselenide (-SeSeR'Y', -SeSeRY), selenide (SeR'Y', -SeRY), thiol (-SH), isonitrile, nitro (-NO.sub.2), selenol (-SeH), trivalent phosphorus compounds, isothiocyanate, xanthate, thiocarbamate, phosphine, thio acid and dithio acid (-COSH, -CSSH) where R and R' are hydrocarbon chains optionally interrupted by hetero atoms, of a length exceeding 10 atoms, wherein in the case of an asymmetrical molecule, R' or R may be H, and Y and Y' are active groups for covalently binding ligands or a biocompatible porous matrix.
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