Stable hydrophilic acridinium esters suitable for liposome encapsulation
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United States of America Patent
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Jul 13, 1993
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Jan 22, 1992
filing date -
Aug 1, 1988
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Abstract
A method for the detection of an analyte in a fluid sample using liposomes encapsulating acridinium esters (lumisomes). Hydrophilic polysubstituted aryl acridinium esters are useful as chemiluminescent markers and can be encapsulated within liposome vesicles without significant leakage of the esters from the vesicles. The lumisomes can be coupled to molecules with biological activity, such as antigens, antibodies, and nucleic acids, and used in luminescent assays.
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