Detection of analytes having binding sites for at least two binding moieties

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United States of America Patent

PATENT NO 5223393
SERIAL NO

07537905

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Abstract

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The present invention provides reagents for a detectable label for use in specific binding assays. Specifically, modified .beta.-galactosidase enzyme donors (ED) and acceptors (EA) are utilized. Both ED and EA are modified to form separate ED and EA complexes by coupling each with a linking element and a binding moiety which is specific to a binding site in the analyte. The ED and EA complexes are incapable of forming active enzyme in the absence of the analyte. However, when analyte is present, ED and EA complexes which bind to a common analyte in a sample, active .beta.-galactosidase is formed.

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Patent Owner(s)

Patent OwnerAddress
MICROGENICS CORPORATION2380A BISSO LANE CONCORD CALIFORNIA 94520

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Khanna, Pyare Fremont, CA 39 1384
Loor, Reuyming Danville, CA 1 21

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