Use of bacterial luciferase structural genes for cloning and monitoring gene expression in microorganisms and for tagging and identification of genetically engineered organisms
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United States of America Patent
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Jun 22, 1993
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Jul 19, 1989
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Jul 22, 1986
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Abstract
A host microorganism is genetically and stably modified by the insertion into any of its non-essential chromosomal location of a non-homologous, recombinant foreign DNA fragment, maintaining an insertion of a luxAB gene of a selected bioluminescent bacterium such as V. harveyi, such that the expression of the luxAB genes causes the production of a luciferase enzyme which, in turn, catalyzes a light-emitting reaction in the presence of the appropriate substrate. X-ray film can be used to quantify the light being emitted from a microorganism through the use of plural droplets containing the same microorganism, each with a known and related cell (or plasmid) count.
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