Method of immobilizing nucleic acid on a solid surface for use in nucleic acid hybridization assays
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United States of America Patent
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Jun 1, 1993
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N/A
app pub date -
Nov 30, 1989
filing date -
Nov 30, 1989
priority date (Note) -
Expired
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Abstract
Methods of immobilizing nucleic acid on a solid surface for us in nucleic acid hybridization assays is disclosed. The methods of the invention comprise reacting a modified nucleic acid strand comprising a variable portion and an anchor portion wherein the variable portion comprises a nucleotide sequence having a selected base sequence and the anchor portion comprises at least one nucleotide base modified with a primary amine function or nucleotide base equivalent having a primary amine function and reacting the modified nucleic acid strand with a free aldehyde group of the solid surface in the presence of a reducing agent to form complexes of the modified nucleic acid strand and at least a portion of the free aldehyde groups on the solid surface.
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