Method for assaying aliphatic alcohol, aliphatic aldehyde or .omega.-carboxylic acid derivatives thereof
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United States of America Patent
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Apr 27, 1993
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Nov 5, 1991
filing date -
Jul 24, 1989
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Abstract
A method for assaying aliphatic alcohol, aliphatic aldehyde or .omega.-carboxylic acid derivatives thereof, using a novel .omega.-carboxyalcohol oxidase which catalyzes at least one of the following reactions: a) R--CH.sub.2 OH+O.sub.2 .fwdarw.R--CHO+H.sub.2 O.sub.2 b) R--CHO+O.sub.2 +H.sub.2 O.fwdarw.R--COOH+H.sub.2 O.sub.2 wherein R is alkyl, alkenyl, .omega.-carboxyalkyl or .omega.-carboxyalkenyl. The enzyme has substrate specificity on at least 12-hydroxydodecanoic acid, 1-dodecanol, 1-decanol, 1-octanol and 1-hexanol, and has no substrate specificity on methanol, ethanol or glycerol. The enzyme does not require the presence of NAD or NADP for its use. Also disclosed is a process for producing the enzyme, an assay method for substrates of the enzyme, and a process for producing carboxylic acid employing the enzyme.
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