Expression of recombinant polypeptides with improved purification
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United States of America Patent
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Apr 13, 1993
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N/A
app pub date -
Aug 7, 1990
filing date -
Aug 7, 1990
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Expired
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Abstract
An improved method for expressing peptides as fusion proteins, uses a carrier for a heterologous peptide to provide a fusion protein having a high pI. The high isoelectric point facilitates separation of the fusion protein from all other host cell proteins, and separation of the carrier from the peptide after cleavage.
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