Process for preparation of activated protein C by immobilized aprotinin chromatography
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United States of America Patent
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Mar 30, 1993
Grant Date -
N/A
app pub date -
Jan 9, 1991
filing date -
Apr 12, 1989
priority date (Note) -
Expired
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Abstract
The present invention relates to a method for preparing activated protein C (APC) from a sample containing the said activated protein C, characterized in that the activated protein C is bound to insolubilized aprotinin and then in that, after washing of the said activated protein C/aprotinin complex with a buffered saline solution, the said activated protein C is collected by elution with an acidic aqueous solution or a solution containing a chaotropic agent. The invention also relates to the activated protein C thereby obtained according to the method described above.
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