Preparation of a blood platelet lysate for use in a cell culture medium for hybridoma cells
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United States of America Patent
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Mar 30, 1993
Grant Date -
N/A
app pub date -
Dec 26, 1990
filing date -
Apr 26, 1988
priority date (Note) -
Expired
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Abstract
A platelet lysate is produced from plasma derived from animal whole blood that contains an added citrate to prevent coagulation of the blood during storage. The lysate is prepared by centrifuging plasma to produce a platelet rich paste, adding calcium to the paste to lyse the platelets therein and coagulate fibrinogen to produce a clear liquid containing lysed platelets, sterile filtering the liquid and collecting a liquid filtrate containing the lysed platelets. The obtained lysate of platelets can be used to wholly or partly replace foetal calf serum in cell culture, such as in culture of hybridoma cells for preparing monoclonal antibodies.
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