Stabilized proteolytic solution and reagent kit
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United States of America Patent
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Mar 9, 1993
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N/A
app pub date -
Dec 18, 1990
filing date -
Dec 18, 1990
priority date (Note) -
Expired
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Abstract
A stabilized proteolytic solution has been developed which can be used to treat selected cells, especially red blood cells. A preferred stabilized solution of the invention comprises about 0.1% to about 0.3% ficin in a citrate-buffered saline solution containing mannitol, L-cysteine, dithiothrietol in an active concentration of less than about 10 mM, and EDTA. A kit for qualitatively identifying unexpected blood group antibodies comprising a series of untreated and ficin-treated red blood cells, a stabilized ficin reagent, a red blood cell diluent and an enzyme control is also provided.
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