T-DNA promoters

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United States of America Patent

PATENT NO 5182200
SERIAL NO

06725368

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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The sequence of the T.sub.L -DNA of Ri plasmids found in Agrobacterium rhizogenes strains HRI and A4 is disclosed. Sixteen open reading frames bounded by eukaryotic promoters, ribosome binding sites, and polyadenylation sites were found, five of which were observed to be transcripted in a developmentally and phenotypically regulated manner. The use of promoters and polyadenylation sites from pRi T.sub.L -DNA to control expression of heterologous foreign structural genes is taught, using as examples the structural genes for Phaseolus vulgaris storage protein (phaseolin), P. vulgaris lectin, a sweet protein (thaumatin), and Bacillus thuringiensis crystal protein. Vectors useful for manipulation of sequences of the structural genes and T-DNA are also provided.

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Patent Owner(s)

Patent OwnerAddress
MYCOGEN PLANT SCIENCE INC5501 OBERLIN DRIVE SAN DIEGO CA 92121

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Slightom, Jerry L Kalamazoo, MI 20 388
Tepfer, David A Paris, FR 5 19

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