Lysing reagent for leukocyte differentiation method
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United States of America Patent
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Jan 19, 1993
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N/A
app pub date -
Jul 5, 1988
filing date -
Jul 5, 1988
priority date (Note) -
Expired
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Abstract
A lysing reagent is employed for stromatolysing the red cells in a blood sample and differentiating the leukocytes into subpopulations. The lysing reagent contains a dodecyltrimethylammonium halide and an aromatic second cationic agent which is either a benzyltrialkylammonium halide or an alkylpyridinium halide. In some methods, the lysing reagent is mixed in the reaction chamber with a major proportion of the diluent, and the blood sample with a minor proportion of the diluent is added to the reaction chamber.
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