Expression and diagnostic use of gag encoded peptides which are immunologically reactive with antibodies to HIV
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United States of America Patent
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Dec 29, 1992
Issued Date -
N/A
app pub date -
Jul 28, 1989
filing date -
Aug 7, 1985
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Abstract
Immunologically reactive gag proteins of LAV are expresesed in bacterial cells. The gag proteins are encoded by a recombinant plasmid containing procaryotic transcriptional and translational signals for expression, followed downstream by a DNA sequence comprising a portion of the gag region of LAV. Preferred signals for expression are selected from an inducible and/or suppressible operon, such as the trp operon. The gag proteins are isolated from the bacterial host and are utilized in diagonstic assays which detect the presence of LAV antigens or antibodies immunologically reactive with LAV. Further, the proteins produced by the method disclosed may be used as a vaccine against infection by the caustive virus for acquired immune deficiency syndrome.
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