Apparatus for pretreating cells for flow cytometry
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United States of America Patent
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Dec 1, 1992
Grant Date -
N/A
app pub date -
Sep 11, 1990
filing date -
Sep 13, 1989
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A plurality of open-top sample tubes, each containing a simple liquid containing cells to be treated, are removably mounted on a disklike sample carrier which is rotatable about a vertical axis for centrifuging the samples. Disposed above the sample carrier are a set of fixed reagent supply nozzles for dropping a desired reagent or reagents into the sample tubes and at least one supply-discharge nozzle movable into and out of the successive sample tubes. A hydropneumatic circuit is coupled to the supply-discharge nozzle for intimately intermingling the samples and the reagents and for rinsing the supply-discharge nozzle and other required parts. In an alternate embodiment a single supply-discharge nozzle is employed for reagent supply into the sample tubes, for intermingling the samples and the reagents, and for rinsing.
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