In vitro propagation of embryonic stem cells
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United States of America Patent
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Nov 24, 1992
Grant Date -
N/A
app pub date -
May 31, 1990
filing date -
Aug 4, 1988
priority date (Note) -
Expired
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Abstract
The present invention relates generally to the use of leukaemia inhibitory factor (LIF) in the maintenance and derivation of embryonic stem (ES) cells in culture. The ES cells are maintained and/or derived from animal embryos by culturing said cells or embryos in a culture medium containing an effective amount of LIF for a time and under conditions sufficient to maintain and/or derive said ES cells. The ES cells may be passaged in LIF and used to make chimaeric animals.
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