Method for diagnosing periodontal diseases with a substrate specific for aminopeptidase activity of periodontopathic bacteria
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United States of America Patent
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Aug 11, 1992
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app pub date -
Dec 29, 1989
filing date -
Jul 29, 1986
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Abstract
A method for diagnosing contraction or progress of periodontal diseases is carried and by determining periodontopathic bacteria specific aminopeptidase activity in a specimen by using as a substrate for the enzyme. The substrate is either or both compounds of the formula: X--Z--Arg--Y [1] wherein Arg is arginine residue; X is or an amino blocking group; Y is a color developing group attached to the C-terminal of Arg; and Z is an amino acid or peptide residue composed of 1 or 2 amino acids or their blocked derivatives, the C-terminal of which is attached to the N-terminal of Arg, and X'--Z'--Pro--Y' [2] wherein Pro is proline residue; X' is an amino blocking group; Y' is a color developing group attached to the C-terminal of Pro; and Z' is an amino acid or peptide residue composed of 0 to 4 amino acids or their blocked derivatives, the C-terminal of which is attached to the N-terminal of Pro.
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