In-vitro methods for identifying compositions which are agonists and antagonists of androgens
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United States of America Patent
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Aug 4, 1992
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app pub date -
Apr 18, 1989
filing date -
Apr 17, 1986
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Abstract
The present invention provides general protocols by which a substance may be identified and characterized as an androgen agonist and/or an androgen antagonist. The described protocols are in-vitro methods which utilize androgen dependent cells in culture and a medium comprising an inhibitor endogenous to the sera of humans and animals which is able to prevent the proliferation of these cultured cells in-vitro. The methodology is rapid, reproducible, and accurate; and provides the major advantage of being able to test large numbers of unevaluated substances for their androgen agonistic and/or antagonistic properties as primary properties or secondary side-effects.
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