Pulsed field gel electrophoresis of large DNA
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United States of America Patent
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Aug 4, 1992
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N/A
app pub date -
May 5, 1989
filing date -
May 5, 1989
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Expired
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Abstract
An electrophoresis apparatus 10 has a power supply 12, an electrophoresis gel system including the tank 85 and an adjustable switching means 42 connected together for controlling the electric field force, electric field angle and the pulse duration to resolve DNA molecules greater than 1,000 kb in length along straight, unbent lanes within a gel. The electrophoresis system 10 permits adjustment of the pulse durations through a range from generally one-tenth of a second to shorter ranges. It permits the adjustability of the electric field force, electric field angle and the pulse duration so that it may have: (1) pulse durations in a range that maintains the pulse durations shorter than one-tenth of a second; (2) an angle or angles of two fields up to 180 degrees with respect to each other; (3) a side range of intensities of the fields; and (4) any number of repetitions of pulses of the fields before changing the angle of the two fields with respect to each other.
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