Method of quantitative determination utilizing enzyme aggregation
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United States of America Patent
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Jul 14, 1992
Issued Date -
N/A
app pub date -
Aug 28, 1989
filing date -
Jan 14, 1982
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Expired
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Abstract
A quantitative determination of a substance is performed in a homogeneous system based on a change in enzyme activity; differences between the activity of a free enzyme and that of an enzyme bound by an aggregation or chemical bonding are observed. A peroxidase-labeled antibody and antigen system is one of of the typical example. Since the reaction is effected in a homogeneous system, the amount of antigen can be easily measured by the difference of enzyme activity.
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