Integration vector for Bradyrhizobium japonicum
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United States of America Patent
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Jun 23, 1992
Grant Date -
N/A
app pub date -
Oct 7, 1987
filing date -
Oct 7, 1987
priority date (Note) -
Expired
status (Latency Note)
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Abstract
An integration vector which directs the stable integration of a DNA segment, including for example, a cloned structural gene and/or a selectable marker into a defined, nonessential region of the Bradyrhizobium japonicum genome is provided. The integration of the DNA occurs via homologous recombination between the DNA sequences which flank the DNA segment and identical sequences in the genome. The integration site is preferably the region of the B. japonicum genome flanked by the sequences RS.alpha.9 and RS.beta.3, near nifDK which is a nonessential region of the chromosome.
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