Process for recovering polypeptides localized in the periplasmic space of yeast without breaking the cell wall by using an non-ionic detergent and a neutral salt
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United States of America Patent
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Jun 23, 1992
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N/A
app pub date -
Nov 5, 1990
filing date -
Nov 13, 1985
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Expired
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Abstract
The invention relates to a process for releasing into aqueous medium polypeptides produced by yeasts and localized at least partially within the periplasmic space thereof without breaking the yeast cell. The process involves treating the yeasts in aqueous medium with a neutral water-soluble mineral salt and a non-ionic water-soluble polyethoxylated alkylphenol surfactant having an HLB of between 8 and 15.
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