Oligonucleotides possessing a primary amino group in the terminal nucleotide
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United States of America Patent
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Jun 2, 1992
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app pub date -
Feb 27, 1991
filing date -
Dec 20, 1983
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Abstract
The invention consists of compounds and methods for the synthesis of oligonucleotides which contain one or more free aliphatic amino groups attached to the sugar moieties of the nucleoside subunits. The synthetic method is versatile and general, permitting amino groups to be selectively placed at any position on oligonucleotides of any composition or length which is attainable by current DNA synthetic methods. Fluorescent dyes or other detectable moieties may be covalently attached to the amino groups to yield the corresponding modified oligonucleotide.
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